Journal: PloS one

Article Title: Menstrual blood as a potential source of endometrial derived CD3+ T cells.

doi: 10.1371/journal.pone.0028894

Figure Lengend Snippet: Figure 6. CMV-specific T cells derived from the menstrual blood. A. PBMC B. MBC from the same healthy woman (#1). Cells were stimulated with overlapping peptides from CMV pp65 and PMA/I as a positive control. Both IFN-c and TNF-a secretion are shown. Percent of cytokine positive cells (IFN-c or TNF-a) are shown above the gate. doi:10.1371/journal.pone.0028894.g006

Article Snippet: CMV-pp65 peptide pool (69 18-mers, overlapping by 10 spanning the entire amino acid sequence of the HCMV pp65 protein, New England Peptide, Gardner, MA) (2 mg/ml) or CMV-Lysate (Advance Biotechnologies, Columbia, MD) (2 mg/ ml) was used to stimulate T cells.

Techniques: Derivative Assay, Positive Control

Journal: PloS one

Article Title: Menstrual blood as a potential source of endometrial derived CD3+ T cells.

doi: 10.1371/journal.pone.0028894

Figure Lengend Snippet: Figure 7. Reduced frequency of CMV-specific T cells derived from the menstrual blood. PBMC and MBC from the same women were stimulated with overlapping peptides from CMV pp65 or CMV lysate and stained for IFN-c. Individual symbols represent paired samples from the same individual. Open symbols represent CD8 T cell responses, closed symbols represent CD4 T cell responses and gray symbols are responses from the healthy volunteer. Statistical compar- isons were made using Wilcoxon Signed Rank test. doi:10.1371/journal.pone.0028894.g007

Article Snippet: CMV-pp65 peptide pool (69 18-mers, overlapping by 10 spanning the entire amino acid sequence of the HCMV pp65 protein, New England Peptide, Gardner, MA) (2 mg/ml) or CMV-Lysate (Advance Biotechnologies, Columbia, MD) (2 mg/ ml) was used to stimulate T cells.

Techniques: Derivative Assay, Staining

Journal: Medicine

Article Title: Human cytomegalovirus in high grade serous ovarian cancer possible implications for patients survival

doi: 10.1097/MD.0000000000009685

Figure Lengend Snippet: Detection of HCMV-IE and pp65 in HGS ovarian carcinoma tissue sections. HCMV-IE and pp65 were detected frequently at different levels in tumor cells and in the stroma in HGS ovarian cancer tissue sections obtained at DEBPC and IDS after NACT. DEBPC = diagnostic excisional biopsy prechemotherapy, HCMV-IE = human cytomegalovirus immediate-early protein, HCMV-pp65 = human cytomegalovirus tegument protein, HGS = high grade serous ovarian carcinoma, IDS = interval debulking surgery, NACT = neoadjuvant chemotherapy.

Article Snippet: Monoclonal antibodies against HCMV immediate-early protein (HCMV-IE) (Chemicon International, MA) and HCMV tegument protein pp65 (BioGenex, CA) were used for the detection of different HCMV proteins and antibodies against keratin 20 (Chemicon) or omitting primary antibodies were used as controls.

Techniques: Diagnostic Assay